Recombinant Antibodies

Recombinant antibodies: highly reproducible with tailored specificity

Making an antibody using recombinant technology offers benefits around consistency, specificity, and scalability.

Recombinant antibodies are manufactured by cloning the immune-specific heavy and light antibody chains into a high-yield mammalian expression vector. The resulting vectors are introduced into expression hosts (eg bacteria, yeast or mammalian) for the manufacturing of high-quality functional antibodies.

Recombinant antibodies can be constructed in vitro by forming an Ig-framework through cloning of ScFV or Fab or can be produced from an existing hybridoma.  In hybridoma-based recombinant antibody generation, mouse, rat, and rabbit models are commonly used. However, as long as the appropriate oligonucleotide primers are available, recombinant antibodies can be developed from any species.

Abcam ID Target Applications Species Reactivity
ab32042 active Caspase-3 ICC/IF, IHC-Fr, WB Hu
ab81283 AKT1 ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab52492 ALDH1A1 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab108337 Alkaline Phosphatase, Tissue Non-Specific Flow Cyt, ICC, IHC-P, IP, WB Hu, Ms, Rat
ab32575 alpha smooth muscle Actin Flow Cyt, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, WB Hu, Ms, Rat
ab124964 alpha smooth muscle Actin Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab52866 alpha Tubulin Flow Cyt, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, IP, WB Dm, Hu, Ms, Pig, Rat
ab138501 Alpha-synuclein ELISA, Flow Cyt, ICC/IF, IHC-P, IP, WB Dm, Hu
ab51253 Alpha-synuclein ICC/IF, IHC-P, WB Hu, Ms, Rat
ab168381 Alpha-synuclein WB Hu
ab209538 Alpha-synuclein filament Dot, ICC/IF, IHC-FoFr, IHC-P Hu, Ms, Rat
ab32047 AMPK alpha 1 Flow Cyt, ICC/IF, IHC-P, IP, WB AGMk, Hu, Ms, Rat
ab133448 AMPK alpha 1+AMPK alpha 2 WB Dm, Hu, Ms, Rat
ab32136 Amyloid Precursor Protein Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB Hu, Ms, Rat
ab133273 Androgen Receptor ICC/IF, IHC-P, WB Hu, Ms, Rat
ab198394 Androgen Receptor (AR-V7 specific) IHC-P, WB Hu
ab108327 APG5L/ATG5 ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab184909 ATF-4 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab81292 ATM Flow Cyt, IHC-P, IP, WB Hu, Ms
ab32420 ATM Flow Cyt, ICC/IF, IHC-P, WB Hu
ab32503 Bax IHC-P, IP, sELISA, WB ChHm, Hu, Ms, Rat
ab32124 Bcl-2 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab182858 Bcl-2 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms
ab32370 Bcl-XL Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab108319 BDNF ICC/IF, IHC-P, WB Hu, Ms, Rat
ab207612 Beclin 1 ICC/IF, IP, WB Hu, Ms, Rat
ab32572 beta Catenin ICC/IF, IHC-Fr, IHC-P, IP, WB AGMk, Cow, Hm, Hu, Mcq, Ms, Rat, Shp
ab128874 Brd4 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab110641 BRG1 ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab46794 Calponin ICC/IF, IHC-Fr, IHC-P, WB Dog, Hu, Ms, Pig, Rat
ab92516 Calreticulin Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Mk, Ms, Rat
ab108362 Caspase-1 IHC-P, WB Hu, Rat
ab32351 Caspase-3 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Rat
ab133357 CD11b IHC-P, WB Hu, Ms, Pig, Rat, RMk
ab52478 CD11b Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IP, WB Hu
ab52632 CD11c Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu
ab75769 CD146 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab182422 CD163 IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab76533 CD31 IHC-P, WB Hu
ab81289 CD34 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Dog, EliAB, Hu, Ms, Pig, Rat, Shp
ab133625 CD36 IHC-P, IP, WB Gpig, Hu, Ms, Rat
ab183685 CD4 IHC-Fr, IHC-P, IP, WB Ms
ab133616 CD4 Flow Cyt, ICC/IF, IHC-P, WB Hu
ab51037 CD44 Flow Cyt, IHC-Fr, IHC-P, WB Hu
ab40763 CD45 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu
ab134045 CD63 IHC-P, WB Hu
ab53004 CD86 Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB CynMk, Hu, Rat
ab92726 CD9 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab32147 Cdk2 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab108349 CDKN2A/p16INK4a Flow Cyt, ICC/IF, IHC-P, IP, WB Hu
ab76541 CDX2 Flow Cyt, ICC/IF, IHC-P, WB Hu, Rb
ab32358 CEBP Beta EMSA, Flow Cyt, ICC/IF, IP, WB Hu, Ms, Rat
ab32064 Cleaved PARP1 IHC-P, WB Hu, Ms, Rat
ab32072 c-Myc Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab138492 Collagen I IHC-P, WB Cow, Hu, Rat
ab32096 CREB Dot, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab32515 CREB Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab91654 CRTC3 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab124824 CXCR4 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab134175 Cyclin D1 ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab133504 Cytochrome C ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab108624 Cytochrome P450 3A5 IHC-P, IP, WB Hu
ab76318 Cytokeratin 10 ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab52625 Cytokeratin 19 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms
ab52635 Cytokeratin 5 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms
ab181598 Cytokeratin 7 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab53280 Cytokeratin 8 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab32362 Desmin Flow Cyt, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, WB Gpig, Hu, Ms, Rat
ab40772 E Cadherin Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu
ab40815 EGFR ICC/IF, IHC-FoFr, IHC-P, WB Hu, Ms
ab32157 EIF2S1 Dot, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB AGMk, Dm, Hu, Ms, N cra, Plnts, Rat
ab134182 ErbB 2 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu
ab92513 ERG ChIP, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab32063 Estrogen Receptor alpha ChIP, Flow Cyt, ICC/IF, IHC-P, WB Dog, Hu, Ms, Rat
ab81298 FAK ICC/IF, WB Hu, Ms, Rat
ab40794 FAK Flow Cyt, ICC/IF, IHC-P, IP, WB Cow, Hu, Ms, Rat
ab108422 FOXA2 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab181602 GAPDH Flow Cyt, ICC/IF, IHC-P, IP, WB Chk, Fsh, Hu, Mk, Ms, Rat, Rb, Xp, Zfsh
ab75836 GCN2 WB Hu
ab183734 GFP Flow Cyt, ICC/IF, IHC-P, WB  
ab115730 Glucose Transporter GLUT1 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab125066 Glutathione Peroxidase 4 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab52649 GM130 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB AGMk, Cow, Dog, Hu, Mk
ab68477 Heme Oxygenase 1 Flow Cyt, ICC/IF, IP, WB Hu, Ms, Rat
ab51608 HIF-1 alpha Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab179483 HIF-1 alpha ICC/IF, WB Hu, Ms
ab81299 Histone H2A.X ChIP, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab52946 Histone H3 ChIP, ICC/IF, IHC - Wmt, IHC-P, IP, WB Ce, Dm, Hu, Rat, S pom, Sc
ab32356 Histone H3 ChIP, CHIPseq, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Chk, Cow, Dm, Hu, Mk, Ms, Rat, Rice
ab52922 HLA A Flow Cyt, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, In-Cell ELISA, IP, WB Hu, Rat
ab79823 HMGB1 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab174830 HMGCR IP, WB Hu, Ms, Rat
ab109489 Human IgG IHC-P, WB CynMk, Hu
ab178846 Iba1 IHC-P, WB Hu, Ms, Rat
ab178847 Iba1 ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab32518 IKB alpha Flow Cyt, ICC/IF, IHC-P, IP, WB Hm, Hu, Ms, Rat
ab178945 iNOS ICC/IF, IP, WB Hu, Ms
ab150361 Integrin alpha 5 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab129202 Integrin alpha E IHC-P Hu
ab179475 Integrin alpha V ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab52971 Integrin beta 1 IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab124945 IRE1 WB Hu
ab76493 IRF3 WB Hu
ab109517 Islet 1 Flow Cyt, ICC/IF, IP, WB Chk, Hu, Ms, Rat
ab32101 JAK2 Dot, Flow Cyt, ICC, IHC-Fr, IHC-P, IP, WB Hu, Ms, Rat
ab124956 JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Flow Cyt, ICC, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab92742 Ki67 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu
ab133741 Lamin B1 ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab192890 LC3B ICC/IF, IP, WB Hu, Ms, Rat
ab52818 LDL Receptor Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms
ab137872 LEF1 Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, IP, WB Hu, Ms, Rat
ab92544 LRP1 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Ms, Pig, Rat
ab133450 LRRK2 ICC/IF, WB Hu, Ms
ab133474 LRRK2 ICC/IF, IHC-FrFl, IHC-P, IP, WB Hu, Ms, Rat
ab203181 LRRK2 WB Hu
ab108508 Lysozyme ICC/IF, IHC-P, WB Hu, Ms
ab32087 MCL1 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab51067 Met (c-Met) Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab195352 METTL3 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Ms, Rat
ab196436 MLKL Dot, IP, WB Ms
ab187091 MLKL IHC-P, WB Hu
ab51074 MMP14 IHC-P, IP, WB Hu, Ms, Rat
ab92536 MMP2 Flow Cyt, ICC/IF, WB Hu, Ms, Rat
ab52915 MMP3 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab76003 MMP9 ELISA, Flow Cyt, ICC/IF, IHC-P, WB Hu, Rat
ab92471 MSH6 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab32028 mTOR ChIP, IHC-P, IP, WB Hu, Ms, Rat
ab109268 mTOR IHC-P, WB Hu, Ms, Pig, Rat
ab76011 N Cadherin IHC-P, WB Hu, Ms, Rat
ab133303 NADPH oxidase 4 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab109250 Nanog Flow Cyt, ICC/IF, IHC-P, WB Hu, Rat
ab177487 NeuN Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Cat, CynMk, Dog, Goat, Hu, Mmst, Ms, Pig, Rat, Shp, Zfsh
ab81321 Neuropilin 1 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Mk, Mmst, Ms, Rat
ab32536 NF-kB p65 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab52627 Notch1 Flow Cyt, ICC/IF, IHC-P, WB Cow, Hu, Ms, Rat
ab129068 NOX2/gp91phox WB Hu, Ms, Rat
ab62352 Nrf2 ChIP, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Ms
ab76026 Nrf2 Flow Cyt, ICC/IF, IHC-P, WB Hu
ab167161 Occludin IP, WB Hu, Ms, Rat
ab181557 Oct4 ChIP, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Ms
ab109186 Olig2 IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab170904 P Glycoprotein IHC-FoFr, IHC-P, WB Hu, Ms, Rat
ab51243 p16 ARC ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab109199 p21 WB AGMk, Hu, Ms, Rat
ab109520 p21 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu
ab32034 p27 KIP 1 Flow Cyt, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB Hu, Rat
ab124762 p63 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat, Rb
ab52987 p75 NGF Receptor Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab32084 Paxillin Flow Cyt, ICC/IF, IHC-P, IP, WB Cow, Dog, Hu, Ms, Rat
ab109497 PBR Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab92552 PCNA Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab137132 PD1 IHC-P Hu
ab32570 PDGF Receptor beta Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, IP, WB Hu, Ms, Rat
ab209960 PD-L1 Flow Cyt, ICC/IF Hu
ab205921 PD-L1 Flow Cyt, ICC/IF, IHC-P, WB ChHm, Hu
ab213524 PD-L1 ICC/IF, IHC-P, IP, WB Hu
ab32376 PKC alpha Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Gfsh, Hu, Ms, Pig, Rat
ab110638 PMS2 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu
ab51257 Polyethylene glycol ELISA, IHC-P, WB  
ab53449 Polyethylene glycol ELISA, IHC-P, WB  
ab109451 PRMT5 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab179515 pro Caspase1 + p10 + p12 IP, WB Hu, Ms, Rat
ab32199 PTEN IHC-P, WB Hu, Ms, Rat
ab137029 RAB7 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab133534 Rad51 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab195117 RIP3 ELISA, WB Ms
ab52642 S100 beta ICC/IF, IHC-P, IP, WB Goat, Hu, Mcq, Ms, Rat, Zfsh
ab92446 SATB2 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab52903 Smad3 ELISA, ICC/IF, IHC-Fr, IHC-P, WB Dm, Hu, Ms
ab40854 Smad3 Flow Cyt, ICC/IF, IHC-P, sELISA, WB Hu, Ms, Rat
ab40759 Smad4 IHC-P, WB Hu, Ms, Rat
ab76020 Sodium Potassium ATPase Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat, Tlp
ab134152 Somatostatin Receptor 2 IHC-Fr, IHC-P, IP, WB Hu, Ms, Rat
ab92494 SOX2 ICC/IF, IHC - Wmt, IHC-P, sELISA, WB Hu, Leucor eri, Ms
ab185966 SOX9 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Pig, Rat
ab185230 SOX9 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab109012 SQSTM1 / p62 Flow Cyt, ICC/IF, WB Hu, Ms, Rat
ab76315 STAT3 ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab68153 STAT3 ICC/IF, IHC-P, WB Hu, Ms, Rat
ab32520 STAT6 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms
ab32058 Sumo 1 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab32127 Synaptophysin ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Dnk, Hu, Ms, Rat
ab109201 TAPA1 WB Hu, Ms, Rat
ab109390 Tau Dot, IHC-P, WB Hu, Ms, Rat
ab210405 TMEM119 Flow Cyt Ms
ab76291 TrkA Flow Cyt, ICC/IF, IHC-P, IP, WB Hu, Ms, Rat
ab181560 TrkA+B+C ICC/IF, IHC-P, WB Hu, Ms, Rat
ab125011 TSG101 Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab76013 TTF1 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat
ab140601 Ubiquitin Flow Cyt, ICC/IF, IHC-P, WB Hu, Ms, Rat
ab179434 Ubiquitin Flow Cyt, ICC/IF, IHC-FoFr, IHC-P, WB Hu, Ms, Rat
ab172730 Rabbit IgG, monoclonal [EPR25A] - Isotype Control CHIPseq, Flow Cyt, ICC/IF, IHC-P  
ab199091 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) Flow Cyt, ICC/IF  
ab199093 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) Flow Cyt, ICC/IF  
ab134047 VCAM1 Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Hu, Ms, Rat
ab32152 VEGF Receptor 1 IHC-Fr, IHC-P, IP, WB ChHm, Hu, Ms, Rat
ab92547 Vimentin Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms, Rat, RMk
ab129002 Vinculin Flow Cyt, ICC/IF, IP, WB Hu, Ms, Rat
ab89901 Wilms Tumor Protein Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Hu, Ms
ab52771 YAP1 Flow Cyt, ICC/IF, IHC-P, IP, WB Hu

 

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